Gel Electrophoresis of Proteins
نویسندگان
چکیده
Gel electrophoresis is a widely known group of techniques used to separate and identify macromolecules as DNA, RNA, or proteins based on size, form, or isoelectric point. The separation of molecules by electrophoresis is based on the fact that charged molecules migrate through a gel matrix upon application of an electric field. These techniques have become a main tool in biochemistry, molecular biology, analytical chemistry and proteomics. Gel electrophoresis is usually used for analytical purposes, but may be a preparative technique to partially purify molecules before applying other techniques, mainly mass spectroscopy to perform proteome analysis (Wasinger et al., 1995).
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